Interleukin 6 polymorphism and acute coronary syndrome

Abstract

Introduction: The polymorphism of interleukin 6 (-174 G>C and – 572 G>C) was considered a modulator of the pathogenesis of various diseases such as cardiovascular diseases and especially acute coronary syndrome (ACS). Therefore, the objective of our study was to assess the relationship between polymorphisms of IL-6 ( -174 G>C and – 572 G>C) and the association between this polymorphism and the acute coronary syndrome in a Tunisian population. Patients and methods: Our study included 264 subjects subdivided into two groups: a group of patients with ACS and a group of apparently healthy controls. The demonstration of the promoter polymorphism of the IL-6 gene (-174 G> C and -572 G> C) was carried out by the polymerase chain reaction (PCR) technique followed by enzymatic digestion, «PCR-RFLP». Results and discussion:The electrophoretic migration profile obtained by PCR-RFLP showed the presence of three genotypes (GG, GC, CC) for each type of polymorphism in the population. Indeed, the wild GG genotype is the most frequent genotype in the two groups of the population and for the two types of polymorphisms, i.e., 76.3% for patients and 83.3% for the controls group for the polymorphism rs1800795 and 82, 3% for patients and 78.9% for controls for the rs1800796 polymorphism. However, it should be noted that there is no statically significant difference in the distribution of genotypes between the two groups studied. Conclusion: We carried out a study to evaluate the relationship between the polymorphisms of IL-6 -174G> C and -572G> C and the development of acute coronary syndrome in a Tunisian population which demonstrated the absence of association between these polymorphisms and the occurrence of ACS.