First case of pouchitis due to Prevotella disiens and Morganella morganii co-infection

Abstract

Objectives: We report a case of pouchitis due to Prevotella disiens and Morganella morganii in a 45-years-old woman patient with ulcerative colitis. Patients and methods : The pus sample was processed for aerobic and anaerobic culture.Identification of anaerobic bacteria and Gram-negative bacilli was realized respectively by ANC and GN cards in VITEK-2 system (BioMerieux, France). Susceptibility results were interpreted using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. Results : After 18–24 h of aerobic incubation on 5% sheep blood agar and Drigalski agar, growth of Morganella morganii was detected. After 72h of anaerobic incubation, very small greyish colonies grew on chocolate agar and were identified as Prevotella disiens. It was susceptible to β-lactams and ciprofloxacin. Conclusion : The majority of anaerobic infections are mixed infections, involving aerobic and anaerobic bacteria that act synergistically. The treatment of anaerobic bacterial infections is complicated because of their long culture and the polymicrobial nature. It must cover anaerobes, enterobacteria and enterococci.

.